On a stake-out: Mycobacterial small RNA identification and regulation

Small interfering RNA; principles, applications and challenges--

Gene silencing using RNAi (RNA interference), has recently been used as a successful laboratory technique in determining the function and control of gene expression and provides a wide range of applications in molecular biology and gene therapy. RNAi is a method of suppressing gene expression. In this direction, a single-stranded RNA molecule of about 21–23 nucleotides, called siRNA (small inte...

RNA Isolation (Small RNA Extraction). Fatemeh Mehrpooyan*

On noise and silence in small RNA regulation

Fluctuations in the number of proteins and RNA molecules can have strong effect on the well-being of a living cell. These molecules are sometimes held in small numbers, and are involved in complex regulatory networks that may amplify small fluctuations. The recently discovered members of the regulatory network, small regulatory RNAs, are sometimes used to keep target mRNAs at exteremely low cop...

SOX2OT, a long non-coding RNA involved in autophagy regulation

Summary: SOX2 overlapping transcript (SOX2OT) is a long non-coding RNA associated with cancer pathogenesis. It contributes to a variety of cellular functions and recent evidence propounds its association with autophagy process. It has been showed that SOX2OT can regulate the expression of different autophagy associated factors in human cells with different mechanisms, however more remains to ...

On Stake and Consensus

In 2009, Satoshi Nakamoto introduced the Bitcoin cryptocurrency[Nak09], an online currency system which allowed peer-to-peer transfer of digital tokens. To ensure a consistent view of token ownership, Nakamoto used a public ledger which can be replicated and validated by all network participants. To avoid a single point of failure, this ledger is authenticated using a dynamic membership multipa...